IL-1 beta anticorps

N° du produit ABIN7126563

L’anticorps Souris Monoclonal anti-IL-1 beta a été validé pour ELISA, IF, IHC (f) et Coat. Il convient pour détecter IL-1 beta dans des échantillons de Humain.

Aperçu rapide pour IL-1 beta anticorps (ABIN7126563)

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-1 beta est non-conjugé

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Détail du produit anti-IL-1 beta anticorps

Specificité: Recognizes a protein of 17-31 kDa, identified as interleukin 1beta. It shows no cross-reaction with interleukin 1alpha. Epitopes of MAb IL1B/463 and IL1B/465 are different and provide a good pair for developing an ELISA. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human IL1B protein

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: LPS stimulated human peripheral blood mononuclear cells. Human colon or liver tissue.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur IL-1 beta

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: IL1B

Sujet: Catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; IL-1 beta; IL1B; IL1F2; Interleukin 1 beta precursor; Interleukin-1 beta; LAF; Osteoclast activating factor (OAF); Preinterleukin 1 beta; Pro interleukin 1 beta,Interleukin-1 beta (IL-1 beta)
Cellular localisation: Cytoplasmic & Extracellular (Secreted)

Poids moléculaire: 31kDa (proprotein), 17kDa (mature)

ID gène: 3553, 126256

UniProt: P01584

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome